Potential Antibacterial Activity of Ethanol Extract of Seagrass (Thalassia hemprichii) From Depapre Jayapura against Propionibacterium acnes

https://doi.org/10.46610/JAPP.2023.v05i02.004

Abstract

Contains of Seagrass (Thalassia hemprichii) are flavonoid compounds, saponins, triterpenoids, alkaloids, steroids, and tannins. These compounds are known to have the potential as antibacterial. This study aimed to determine the inhibition of antibacterial activity and the most effective concentration inhibiting Propionibacterium acnes bacteria. The sample used in this study was seagrass leaves obtained from the Depapre District. Samples were extracted by the maceration method using 96% ethanol solvent. Then, the antibacterial test was carried out using the disk diffusion method. The medium used for bacterial isolation was Nutrient Agar (NA). This study used a completely randomized design (CRD) with concentrations of seagrass leaf extract 50, 100, 200, 400, 600, 800, and 1000 ppm, positive control (clindamycin), and negative control (sterile distilled water). The inhibition zone diameter was analyzed using the ANOVA test. A further test was carried out to find out the difference between the treatments of a significantly honest difference (HSD). The results showed that the inhibition zone diameter in Propionibacterium acnes ranged from 6–10 mm (medium) and 10–20 mm (strong). Based on the results, it was shown that the interaction between the concentration of Thalassia hemprichii and the type of bacteria had a significant effect (P<0.01) on the inhibition zone diameter formed.