The Action of Leaf Extracts of Prunella Vulgaris L. and Ocimum Araratum L. on the Peroxidase Activity of Erythrocytes of Patients with Chronic Obstructive Pulmonary Disease (COPD)

Abstract

Background: Prunella vulgaris and Ocimum Araratum has rosmarinic acid which has antioxidant, anti-inflammatory effects also preventing the formation of reactive oxygen species and lipid peroxidation. Materials/Methods: The active component is extracted from dry crushed leaves of P. vulgaris L. and O. araratum L. in phosphate buffer (pH 7.4, 10 mM) and in 25%, 50%, 70%, 96% ethanol (1 g per 30 ml of extract). For total flavonoid content in the extracts, a colorimetric method was used. Antiradical activity was determined by the restoration of the stable radical DPPH. To determine the major components of a flavonoid nature, high performance liquid chromatography was used. Blood sampling was carried out in healthy donors (the number of donors was 15, all men, among them were from 50 to 60 years old, non-smokers) and in COPD patients in severe exacerbation stage(the number of patients were 15, all men aged 50-60 years old, smokers).The peroxidase (PO) activity of haemolysed blood with extracts was determined. Results: The highest content of RK in a 50% ethanol extract, which is 1.6 times more than 96% ethanol extract and 1.1 times more than 70% same is seen in O.araratum; the highest RK content in 50% ethanol extract, which is 1.5 times higher than in 25% ethanol extract, 2.6 times higher than in 96% ethanol extract and 1.2 times more than 70%.The results of peroxidase activity of healthy donor haemolysis in the presence of different extracts showed not much of a change but remained on higher side. In COPD patients, the presence of extracts showed an additive effect. The most active are 70% ethanol extract of P. Vulgaris and 50% ethanol extract of O.araratum, which correlated with a high content of PK. Conclusions: For therapeutic purposes, 70% ethanol extract of P.vulgaris and 50% ethanol extract of O.araratum can be used to increase the peroxidase activity in both healthy and COPD subjects. But has greater beneficial effects in patients with COPD.