Cytoxic Activity Test of Akway (Drymis Sp) Wood Fraction of From Manokwari, West Papua with BSLT Method

Abstract

Papua has natural wealth that can be used for traditional medicine, one of which is the Akway plant (Drymis sp), a typical plant from Manokwhe people often, West Papua. The part that t as a treatment is the bark of Akway (Drymis sp) utilizing such scraping/grating the bark, then biting or boiling it to drink during long trips to increase stamina. The study aimed to test the cytotoxic activity of the Akway (Drymis sp) bark fraction against Artemia salina shrimp larvae using the BSLT method. The research was carried out in an extraction stage using the maceration method with 96% ethanol solvent and then fractionated using n-hexane, ethyl acetate, and ethanol as solvents. Subsequently, a phytochemical screening test was carried out. A series of concentrations of 10,50,100,250,500 ppm was carried out on the bark fraction of Akway (Drymis sp) for cytotoxic testing of Artemia salina shrimp larvae by obtaining an LC50 value. The results showed that the LC50 value of the Akway bark fraction included the ethyl acetate fraction of 26.30 ppm with the very toxic category, the n-hexane fraction 66.07 ppm, and the ethanol-water fraction 154.88 ppm with the poisonous category.