Overview of Proteomics Techniques and Limitations

Abstract

The investigation of proteins and their interactions within a cell is known as proteomics. The focus is now on the human body's protein makeup following the Human Genome Project's completion. A cell, tissue, or organism's proteome more accurately reflects its dynamic condition; hence proteomics is projected to generate disease markers that are better for diagnosing and monitoring therapy. For those looking to better understand illnesses, the development of proteomics technology for the worldwide identification and quantification of proteins presents new opportunities and problems. Modern bioinformatics and high-throughput proteomics techniques are commonly used to identify disease-related molecular signatures according to protein pathways and signalling cascades. Proteomics frequently makes use of mass spectrometry, a method that has become crucial in molecular and cellular biology. Despite the promise, there are still many issues that need to be overcome, like finding low protein abundance and combining using proteomics genome and metabolomics data. Yet, proteomics offers setting the stage for creating and acquiring pertinent information for biological research. An overview of current problems with proteomics technology is included in this article.