Determination of Flavonoid and Phenolic Levels and Antioxidants Activity Test of Seagrass Halophila ovalis extracts from Jayapura City

Abstract

The utilization of marine life today is not only used as food but has led to more advanced and modern research. So far, many unique active compounds have been isolated from marine biota, and a number of these compounds are in the development stage to become new drug candidates. Like other tropical aquatic organisms, seagrass produces secondary metabolites in the form of antioxidants, so this seagrass is very prospective to be used as a source of medicine and healthy food. This study aims to carry out phytochemical screening and determine the total levels of phenolic and flavonoid compounds and antioxidant tests from the ethanol extract of Halophila ovalis using the DPPH (2,2-diphenylpicrylhydrazyl) method. The research stages started with sample collection and extract-making using the maceration method using 96% ethanol as solvent. The results of phytochemical screening from ethanolic extract of Halophila ovalis obtained from Jayapura city containing Alkaloids, Flavonoids, Tannins, and Triterpenoids/Steroids. The total flavonoid content is 195.882 mg QE/gram, while the average total phenolic content is 61.658 mg GAE/gram. Halophila ovalis ethanol extract has potent antioxidant activity with an IC50 value of 15.226 ppm and an AAI value of more than 2, which are 2.627.