Phytochemical and Pharmacological Effects of Ardisia solanacea Bark on Colon Cancer

Abstract

This study was designed for Phytochemical and pharmacological effect of Ardisia solanacea bark on colon cancer. The main objectives of this study were to investigate the effect of Ardisia solanacea bark as a preventive measure for Dimethylhydrazine induced colon cancer and identification of phytoconstituents. The in-vitro anti-colon cancer activity was evaluated by MTT assay using HT-29 cell line. The Pharmacological in-vivo study was done by using male Wistar rats. Rats were divided into five groups, named - Normal control, DMH control, standard (5-fluorouracil), treatment groups were 100mg/kg and 200 mg/kg. The rats were euthanized at the end of study and a part of colon was examined. The preliminary Phytochemical screening of Ardisia solanacea extract exposed the presence of Flavonoids, Protein & amino acids, Carbohydrates, Saponins, sterols and terpenoids. In column chromatographic technique ethyl acetate fraction of Ardisia solanacea extract gave a compound, by analyzing its physical character and TLC profile it assumed as flavonoid. The extract showed significant anti-colon cancer activity compared with 5-fluorouracil through MTT assay. Aberrant crypt foci (ACF) number had a statistically significant difference in all treatment groups compared to DMH control group in in-vivo pharmacological activity.